An angular magnification (magnifying power) of 30X is desired using an objective of focal length 1.25cm and an eyepiece of focal length 5cm. How will you set up the compound microscope?
1. Given Data
- Total Magnification (M): 30
- Focal Length of Objective (fo): 1.25 cm
- Focal Length of Eyepiece (fe): 5 cm
- Least Distance of Distinct Vision (D): 25 cm
2. Calculating Eyepiece Magnification (me)
When the final image is at the near point, the magnification of the eyepiece is:
me = 1 + D/fe
me = 1 + 25/5
me = 1 + 5 = 6
3. Calculating Objective Magnification (mo)
The total magnification (M) is the product of the individual magnifications:
M = mo × me
30 = mo × 6
mo = 30 / 6 = 5
4. Setup Calculation
Step A: For the Objective Lens
Since mo = vo / |uo| = 5, we have vo = 5|uo|.
Using the lens formula (1/vo - 1/uo = 1/fo) and sign conventions (uo is negative):
1/(5u) - 1/(-u) = 1/1.25
1/5u + 1/u = 1/1.25
(1 + 5) / 5u = 1/1.25
6 / 5u = 1/1.25
5u = 6 × 1.25
5u = 7.5
u = 1.5 cm (Object distance from objective)
Consequently, image distance vo = 5 × 1.5 = 7.5 cm.
Step B: For the Eyepiece Lens
The final image is at D, so ve = -25 cm.
Using the lens formula:
1/ue = 1/ve - 1/fe
1/ue = 1/(-25) - 1/5
1/ue = (-1 - 5) / 25
1/ue = -6 / 25
ue = -25 / 6
|ue| ≈ 4.17 cm
5. Final Setup Configuration
Separation between lenses (L):
L = |vo| + |ue|
L = 7.5 + 4.17
L = 11.67 cm
How to set up the microscope:
- Object Position: Place the object 1.5 cm in front of the objective lens.
- Lens Separation: Adjust the distance between the objective and the eyepiece to be 11.67 cm.
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